applications of electrophoresis slideshare

Gel Electrophoresis Instrument

Gel electrophoresis instruments are used to separate nucleic acids and proteins based on their size and charge Used in forensic molecular biology genetics and microbiology labs gel electrophoresis instruments are used to run and compare DNA samples The two basic types of gel electrophoresis instruments available are horizontal and vertical With the horizontal gel electrophoresis

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DNA sequencing

DNA sequencing technique used to determine the nucleotide sequence of DNA (deoxyribonucleic acid) The nucleotide sequence is the most fundamental level of knowledge of a gene or genome It is the blueprint that contains the instructions for building an organism and no understanding of genetic

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Methods for Protein Purification in Biotechnology

Protein purification is often performed using filters and prepared gel-filtration columns Dialysis Kit Follow the dialysis kit's instructions and add the right volume of the right solution and wait for the specified length of time while collecting the eluant (the solvent passed through the column) in a fresh test tube

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Principle and Protocol of Sodium Dodecyl Sulphate

Tris- glycine electrophoresis buffer: weigh 15 1g Tris and 94g glycine Dissolve in 900ml deionized water then add 50ml 10% (w/v) SDS and deionized water to 1000ml Dilute 5-fold when using The final concentration would be: Tris 25mmol/L glycine 250mmol/L SDS 0 1% and the pH of the buffer is 8 3 8 Polyacrylamide gel electrophoresis tank and electrophoresis power supply 9 Transfer

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Immunoelectrophoresis

After electrophoresis completes 20 μl of the corresponding antiserum is added to troughs in a moist chamber and incubated for 18- 20 hours at room temperature in a horizontal position The agarose gel is placed on a horizontal position and dried with blotter sheets The gel in saline solution is soaked for 10 minutes and the drying and washing repeated twice again The gel is dried at a

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5 Common Dyes for Visualizing and Staining DNA

5 Common Dyes for Visualizing and Staining DNA Share Flipboard Email Print Andrew Brookes / Getty Images Science Biology Genetics It can be used in the gel mixture the electrophoresis buffer or to stain the gel after it is run This makes it very useful for applications like quantitative real-time PCR

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CHAPTER 14 LECTURE NOTES : RECOMBINANT DNA

CHAPTER 14 LECTURE NOTES : RECOMBINANT DNA TECHNOLOGY I General Info A Landmarks in modern genetics 1 Rediscovery of Mendel's work 2 Chromosomal theory of inheritance 3 DNA as the genetic material 4 Recombinant DNA technology development and applications B Recombinant DNA refers to the creation of new combinations of DNA segments that

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Capillary Electrophoresis Guidebook Principles Operation

Apr 05 2020 capillary electrophoresis guidebook principles operation and applications methods in molecular biology Posted By Ian Fleming Media TEXT ID 01025a302 Online PDF Ebook Epub Library metadata downloading covers for books transferring books from just one system to

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Physical Biochemistry: Principles and Applications 2nd

This item: Physical Biochemistry: Principles and Applications 2nd Edition Essentials of Chemical Biology: Structure and Dynamics of Biological Macromolecules (Paperback $110 95) Cannot be combined with any other offers Original Price: $223 90 Purchased together: $167 93 save $55 97 Buy all now! Buy Both and Save 25%! This item: Physical Biochemistry: Principles and Applications 2nd

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Biotechnology Protocols: Rocket immuno

Aim: To perform rocket immuno-electrophoresis (RIEP) for the determination of various concentration of antigen in given unknown sample Principle: Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample Various concentrations of antigen are loaded side by side

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Polyacrylamide Gel Electrophoresis (Theory) : Molecular

PAGE (Polyacrylamide Gel Electrophoresis) is an analytical method used to separate components of a protein mixture based on their size The technique is based upon the principle that a charged molecule will migrate in an electric field towards an electrode with opposite sign The general electrophoresis techniques cannot be used to determine the molecular weight of biological molecules because

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Pulsed

Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA fingerprint for a bacterial isolate A bacterial isolate is a group of the same type of bacteria PulseNet investigates bacterial isolates from sick people contaminated food and

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Proteomics in Cancer Biomarkers Discovery: Challenges

Proteomics in Cancer Biomarkers Discovery: Challenges and Applications Reem M Sallam 1 2 3 1 Clinical Chemistry Unit Department of Pathology College of Medicine King Saud University Riyadh 11461 Saudi Arabia 2 Obesity Research Center College of Medicine King Saud University Riyadh 11461 Saudi Arabia 3 Department of Medical Biochemistry and Molecular Biology Faculty of

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SDS

SDS-PAGE gels: You can prepare your own SDS-PAGE gel or purchase them 'precast' from commercial sources Be sure to select a precast gel that fits well into the electrophoresis chamber Proteins samples: Cell lysates or protein mixture can be diluted 1 to 1 using 2X SDS-PAGE Sample Buffer and boiled for 10 minutes In addition to SDS a reducing agent such as Dithiothreitol (DTT) or 2

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DNA Purification

Figure 3 Agarose gel electrophoresis of PCR products amplified from 1l of mouse tail CHO cells and tomato leaf sample genomic DNA isolated using the Wizard SV 96 Genomic DNA Purification System A total of 10l of PCR product is visualized on a 1 5% agarose gel stained with ethidium bromide Panel A IL-1β (1 2kb) amplified from mouse tail

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Gel Electrophoresis: Basics Steps

Gel Electrophoresis: Basics Steps Purpose: To separate DNA molecules according to their size This can be done for forensic purposes to look for disease in specific genes or paternity testing Can We Write Your Essay? Ace your next assignment with help from a professional writer Free proofreading and copy-editing included Check the Price Hire a Writer Get Help Brief overview: DNA is

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Principle of the PCR

Before the PCR product is used in further applications it has to be checked if : There is a product formed Though biochemistry is an exact science not every PCR is successful There is for example a possibility that the quality of the DNA is poor that one of the primers doesn't fit or that there is too much starting template The product is of the right size It is possible that there is a

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CHAPTER 14 LECTURE NOTES : RECOMBINANT DNA

CHAPTER 14 LECTURE NOTES : RECOMBINANT DNA TECHNOLOGY I General Info A Landmarks in modern genetics 1 Rediscovery of Mendel's work 2 Chromosomal theory of inheritance 3 DNA as the genetic material 4 Recombinant DNA technology development and applications B Recombinant DNA refers to the creation of new combinations of DNA segments that are not found together in nature The

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